Adaptive scanning optical microscope automates lab tasks
DECEMBER 13--An innovative microscope technology invented by researchers at Rensselaer Polytechnic Institute (Troy, NY, USA;www.rpi.edu) has been licensed by Thorlabs (Newton, NJ, USA; www.thorlabs.com, a manufacturer of photonics products. The device, called the Adaptive Scanning Optical Microscope (ASOM), provides the ability to view large areas of a sample without sacrificing image resolution. It is especially suited for automating difficult tasks in biological laboratories, from diagnosing cancer to discovering new drugs.
The technology was invented at the Center for Automation Technologies and Systems (CATS) at Rensselaer by Ben Potsaid, John Wen, and Yves Bellouard, based in part on funding from the US National Science Foundation. Thorlabs has donated equipment to CATS in the past and also actively supports the Smart Optics Laboratory, one of several labs at CATS, where the ASOM research is continuing.
"We are very excited about this license and technology transfer," said Wen, who is also director of the CATS. "We have had an ongoing relationship with Thorlabs, and we look forward to working with them to help accelerate the development of the ASOM to market."
Laboratory workers have benefited from automated microscopes that can outperform human operators. These systems can capture information that can be difficult or tedious for humans, such as tracking moving organisms or processing large numbers of samples automatically. But there is an inherent trade-off between field of view and magnification--as you zoom in on a subject, the amount of area you can see in the viewfinder gets smaller and smaller. This means that large regions of a sample cannot be imaged at high resolutions without moving the sample or microscope, potentially causing undesirable disturbances, according to Potsaid, who also leads the Smart Optics Lab at CATS.
The ASOM overcomes this obstacle by scanning a mirror over the sample, while a camera captures a series of small, distinct snapshots. These images are then assembled into a mosaic, providing a much larger field of view at very high resolution, without the need to switch lenses or move the sample.
The challenge with this method is that when the mirror is not looking straight down onto the sample, it introduces blurriness. CATS researchers fixed this problem by using an adaptive optic element, in this case a "deformable" mirror that changes shape to correct for the off-axis aberrations that cause the blurring. The device, which functions much like a dynamic funhouse mirror, is made up of tiny microelectromechanical systems, allowing the ASOM to operate 10--100 times faster than current automated microscopes without disturbing the specimen.
The ASOM offers other advantages, as well. The microscope can be programmed to quickly scan specific regions of interest in the sample, bouncing back and forth between tracking multiple moving objects. This feature could be useful for observing live microorganisms or in monitoring microscale industrial processes.
Rensselaer's technology will initially be marketed by Thorlabs as a benchtop instrument for biological laboratories and microrobotics research. In the future, it could be used in industrial quality assurance and automated medical diagnostics.